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VOL. 7, ISSUE 2 (2022)
Analysis of genetic similarity and variability of selected varieties and F4 hybrids in Sesame (Sesamum indicum L.) by using RAPD method
Authors
P Arulmurugan, S Jaya kumar
Abstract
Sesame is one of the oldest oil crops known and used by humankind because of its ease of extraction, great stability, and drought resistance. Sesame seed flour has high protein content with high level of the essential amino acids; methionine and tryptophan contain about 10 to 12 per cent of oil and has three times more calcium than milk. Nevertheless the Sesame productivity is low when compared to number of human population in India because of lack of potential varieties in Sesame genotype. In connection with that intraspecific hybridization is one of the easy and successful crossing methods to improve Sesame genotype. The present investigation deals with five varieties of Sesame (TMV-3, TMV-4, TMV-7, VRI-1 and VRI-2) were selected for analysis of genetic similarity and variability between varieties and its hybrids. The hybrids obtained from F4 research field population. Random amplified polymorphic DNA (RAPD) is a PCR based technique for identifying genetic variation and genetic similarities. It involves the use of a single arbitrary primer in a PCR reaction, resulting in the amplification of many discrete DNA products. So, the present research paper was undertaken to analysis of genetic similarity and variability of some selected varieties and its hybrids of Sesame (Sesamum inducum L.) by using RAPD method.
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Pages:292-298
How to cite this article:
P Arulmurugan, S Jaya kumar "Analysis of genetic similarity and variability of selected varieties and F4 hybrids in Sesame (<em>Sesamum indicum </em>L.) by using RAPD method ". International Journal of Botany Studies, Vol 7, Issue 2, 2022, Pages 292-298
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