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VOL. 7, ISSUE 2 (2022)
Isolation of phosphate solubilizing microorganisms and their effect on seed germination and growth of Pisum sativum
Authors
Mini K Paul, Aswathy Shaji, Haripriya S, Nisha Joy
Abstract
The aim of this study was to isolate phosphate solubilizing microorganisms from soil samples in and around MES College, Marampally and evaluate their effect on seed germination and growth of Pisum sativum under constant observation. A total of 23 phosphate solubilizing microorganisms were isolated from 50 samples collected randomly from rhizosphere soil and root nodules. The phosphate solubilizing microorganisms were selected based on their solubilisation index on Pikovskaya’s medium (PVK) and phosphatase assay. Two isolates named as PSM01 and PSM21 showed highest amount of phosphate solubilisation and were selected for further studies. PSM01 and PSM21 were identified as Aspergillus niger and Aspergillus fumigatus respectively. Their inoculation onto Pisum sativum increased the percentage of seed germination (75%), amplified radicle and plumule lengths of germinated seeds, increased mean shoot lengths and root lengths and vigour index (513.75) when compared to non-inoculated ones. On seventh day, plumule and radicle length of A. niger and A. fumigatus treated P. sativum were 3.8 and 4.8 cm respectively. On statistical analysis P value in case of A. fumigatus and A. niger is found to be < 0.05, indicating the effect is extremely significant. These two Aspergillus sp. act as efficient phosphate solubilizer and can be considered as a substitute for chemical fertilizers in agricultural systems, after evaluation of its performance and environmental impact by proper field trial, thereby functioning as an ideal bio fertilizer.
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Pages:353-361
How to cite this article:
Mini K Paul, Aswathy Shaji, Haripriya S, Nisha Joy "Isolation of phosphate solubilizing microorganisms and their effect on seed germination and growth of <em>Pisum sativum</em>". International Journal of Botany Studies, Vol 7, Issue 2, 2022, Pages 353-361
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