Neolitsea sericea, commonly known as Japanese silver tree, is used as herbal medicine for the treatment of inflammation, fever, body pain and diarrhoea. This plant is distributed in the South and Southeast Asia. The present study was aimed at the evaluation of analgesic, anti-inflammatory and antidiarrheal activities of the methanolic crude extract of Neolitsea sericea to authenticate its use in traditional medicine. Methanolic extract of leaves was prepared, and preliminary phytochemical analysis was carried out. In vivo assays were performed in Wistar albino rats to assess the analgesic activity by tail immersion method. Anti-inflammatory activity was evaluated by carrageenan-induced paw edema in rat model while the antidiarrheal activity was determined by charcoal meal test. The results of in vivo experiments revealed that the extract possesses significant analgesic activity at p<0.001 level in a dose dependent manner. Carrageenan-induced paw edema test in rats exhibited 26.95% and 28.95% inhibition of inflammation, respectively, at 200 and 400 mg/ kg b.w. dosage level after 4 h of drug administration. Statistically significant reduction in gastrointestinal transit was observed at 400 mg/ kg b.w. in charcoal meal test. The results of the present investigation proved that Neolitsea sericea is endowed with analgesic, anti-inflammatory and antidiarrheal properties in rats.