Objective: The primary objective of the study is to describe the pharmacognostic nature of the rare medicinal plant which can be considered as anticariogenic agents due to the presence of active constituents in it. Methods: This was carried by collecting the whole plant of Acmella calva (DC.) R.K. Jansen, subjected to successive solvent extraction procedure. Presence of phytochemical constituents, and other pharmacognostic studies likes percentage of moisture content, Ash values, fluorescence analysis and HPTLC analysis were performed. Results: The crude part of selected medicinal plant was subjected to calculate the percentage of moisture content and ash values. The fluorescence analysis showed the presence of flavonoid (yellow), steroid (Brown) and phenols by colour formation at longer and shorter wavelengths. Ethanolic extract of selected medicinal plant was considered to carry out the study because the plant possessed high yield in solvent ethanol at 23% respectively and showed the presence of active phytochemical constituents like tannins, steroids, phenols and flavonoid in the preliminary phytochemical analysis. Then HPTLC fingerprinting was performed using mobile phase (Toluene: Ethyl acetate: Methanol (7:2:1)) which showed the presence of flavonoid with the Rf value 0.54 at 366nm. Conclusion: The present study, indicates the capability of selected medicinal plant for anticariogenic activity due to the presence of good flavonoid content and as they are obtained naturally the chance and range of side effects are considered to be less. Yet pharmacological activity has to be performed for further details.