Assessment on the floristic diversity in Karuppusamy sacred grove near Thathanur in the Ariyalur district of Tamil Nadu, India, was carried out during 2020-2021. In the study, a total of 121 plant species were recorded belonged to 104genera distributed among 50 families, are Angiosperms. Fabaceae (8 species) is a dominant family followed by Asclepiadaceae, Mimosaceae (7 species), Euphorbiaceae, (6 species), and 5 species each from Rubiaceae and Poaceae. The diversity indices namely the Shannon-Weiner index, Simpson index, and evenness index, etc., were analyzed.