Plant Protease inhibitors (PIs) are the mostly studied defense proteins of the Plants. They are highly present in seeds and other storage tissues which represents up to 10% of the total protein (Casaretto and Corcuera 1995). Protease inhibitors carry out some physiological changes in the insect pest by inhibiting their gut tryptic and chymotryptic proteinases. This adversely affect their protein digestion. The insect resorts the overproduction of proteases to compensate for the inhibited activity leading to deficiency of essential amino acids. This exerts additional physiological stress resulting in inhibition of growth. The results obtained in the present study opens new perspectives for utilization of protease inhibitors from the Vigna species in agriculture sector. Total 16 Vigna accessions were studied to find out its tryptic and chymotryptic inhibitory activity. The electrophoretic study of the 16 different Vigna genotypes was performed out of which 3 genotypes found to be the potent tryptic and chymotryptic inhibitors.