Maydis Leaf Blight (MLB) or Southern corn leaf blight (SCLB) caused by Bipolaris maydis (Nisikado) Shoemaker is a serious foliar disease of maize distributed widely in maize producing areas throughout the world where grown under warm and humid conditions. The present study was conducted with the aim of assess the MLB incidence of maize in three districts of Tamil Nadu revealed the endemic nature of the disease with incidence ranging from 5.61 to 26.41 per cent. The maximum per cent disease index (26.41%) was recorded in Keeramangalam village of Pudukkottai district. Maydis leaf blight infected leaf samples were collected and pure hyphal tip cultures of nine isolates were maintained on potato dextrose agar. The pathogenicity of the fungal pathogens was also proved after artificial inoculation of maize plants.