Nanotechnology deals with nanoparticles with sizes ranging from 1-100 nm that are purposely used in healing contact, atomic flow, and other areas. The influence of nanoparticles on the natural environment is causing increasing concern among natural scientists and the general public. Gold nanoparticles are frequently made from compounds that are hazardous and flammable in some way. This review focuses on a biosynthetic and ecologically friendly approach for pharmacological applications of gold nanoparticles derived from Myristica dactyloides fluid extract. UV-Vis spectrometry, Fourier Transform Infrared Spectroscopy (FTIR), Scanning Electron Microscope (SEM) with Energy-Dispersive Spectroscopy (EDX), Dynamic Light Scattering analyses (Particle size and Zeta potential), and X-ray Diffraction have all confirmed the arrangement and characterization of AuNPs (XRD). The antibacterial properties had worn off on a few human pathogenic strains at various doses. The Au nanoparticles successfully inhibited the growth of microbial pathogens in a time-dependent and backbreaker manner.