Cassia tora L is a seasonal weed of the Leguminosae family, commonly reported to have medicinal properties, such as laxative, anti-helminthic, and is effective against leprosy, ringworm, cough, bronchitis, dizziness. of the heart, etc. In the Ayurvedic medicine system, the Cassia tora has a good reputation for all types of skin diseases. In the present study, we attempted to study the HPTLC profile and antibacterial activity of ethanolic extract from the root, stem, leaves, and seeds of the Cassia tora. Chromatographic Analysis (HPTLC) has shown the existence of several polyvalent phytochemical computers with variable Rf values ​​and concentrations. Antimicrobial extract studies have shown significant growth inhibition against E. coli, S. aureus, S. epidermidis S. pyogenes, S. pneumoniae, and P. aeruginosa. A mixture of existing extruded polyvalent compounds may be responsible for antimicrobial activity against bacteria producing redness. The results obtained support the use of C. tora in a few traditional ethnomedicinal systems. In addition, advanced HPTLC fingerprints can detect their effectiveness in accurate C detection. tora and in finding adultery in herbal medicine and in the preparation of herbs.