To know effect of different herbicides on physiological growth parameters of tuberose a experimental trial was laid out at farmer’s field Chinnapudur, Dharmapuri (dit) during 2017-2018 and 2018-2019 seasons. The trial was formulated in randomize block design with ten treatments and replicated thrice. Among different herbicide treatment application of pendimethalin at the rate of 1.0 kg ha-1 on 3 days after planting fb hand hoeing + pendimethalin at 1.5 kg ha-1 on 30, 90, 150, 210 days after planting (T2) increased photosynthetic rate (31.62 and 32.80 µmol m-2 s-1), transpiration rate (22.85 & 24.45 mmol m-2s-1), reduced leaf temperature(28.9 and 28.6ºC). In contract to this unweeded check resulted reduced photosynthetic rate, transpiration rate and increased leaf temperature. All the above mentioned physiological parameters are correlated with flower yield of tuberose.