The mushroom Tricholoma giganteum was collected from Yercaud hills of Salem district in Tamil Nadu. It is the new edible milky mushroom resembles like Calocybe indica which can be grown at high temperature during summer season. Present study was carried out to evaluate the effect of different agro cellulosic wastes for production of the T. giganteum (TGSLM). Among the different substrates tested, paddy straw substrate showed the superior result which recorded minimum days for spawn run (21.55 days), pinhead formation (13.11 days) and for first harvest (19.14 days) with least total number of day for first harvest (40.39 days) and maximum yield of 1698.14 g/bed dry substrate. The highest biological efficiency 169.81% was also obtained in the paddy straw substrate. Followed by banana dry leaf substrate was recorded as the next best superior substrate for cultivation of milky mushroom Tricholoma giganteum.