Lasiodiplodia theobromae causing mango stem end rot is a devastating pathogen which render the mango fruits completely ineffective because it infect the fruit in both field and storage condition. Every living being requires food for its growth and reproduction and the fungi are not an exception. In the present study, potato dextrose agar medium significantly supported the maximum (78.49 mm) growth of L. theobromae followed by beetroot sucrose medium (76.21 mm), carrot sucrose agar (71.95 mm) and bean juice agar (70.72 mm). Among the isolates tested, LT6 from Villupuram had the highest mean mycelial growth of (90.00 mm) followed by LT5 from Palakodu (88.37 mm) and LT3 from Oothangarai (61.07 mm). Among the liquid media tested, potato dextrose broth recorded the highest mean mycelial dry weight (267.11 mg /100 ml) followed by beetroot sucrose broth (256.19 mg/100ml) and isolate LT6 recorded the highest mean mycelial dry weight (268.22mg/100ml), whereas the least mean mycelial dry weight (132.44 mg/100ml) was found in LT2. In general, all the seven isolates grow well on PDA and produced sporulation. Of these, isolate LT6 recorded maximum mycelial growth (90.00 mm) and took 17 days for pycnidial production. The colony colour and shape of conidia in all the isolates were varies slightly and conidial colour varied from brown, golden brown and dark brown. The colony colour of all the isolates varied from greyish white to greyish black. Hence, each and every pathogen requires various culture media for their growth and development.