Plants are essential sources of therapeutic medications and contribute to the survival of local people, tribes, and ethnic groups. Because plants are readily available and less expensive than modern pharmaceuticals, they have been widely employed in the prevention and treatment of various human and animal ailments. Chemical substances were created by all plants. These chemical compounds have the potential to be used as medications, and the scientific basis for their use in modern medicine is the content known pharmacological activity of these molecules in medicinal plants, if properly proven. Ethno medicinal plants are now abundant natural sources of important pharmacological components that are utilized to treat a variety of maladies in humans. The goal of this research is to learn more about the medicinal properties of plants and to raise awareness about its ethnic significance. From February 2021 to February 2022, an ethno-botanical study was done in the Shekhawati region of Rajasthan to chronicle local knowledge of therapeutic plants. Interviews and questionnaires were used to get information from local residents. This study identified 18 plant species belonging to 14 families that are employed by local traditional healers. The plant species used by local people in the study region to treat various ailments.