International Journal of Botany Studies

International Journal of Botany Studies


International Journal of Botany Studies
International Journal of Botany Studies
Vol. 7, Issue 3 (2022)

Meta-topolin influenced shoot morphogenesis in Sansevieria cylindrica Bojer ex Hook. through leaf disc culture


Arjumend Shaheen, Najat A Bukhari, Kahkashan Perveen, Taiba Saeed, Anwar Shahzad

Meta-toplolin (mT) is now considered as a potential phytohormone in the regulation of morphogenesis and other physiological activities in the plant cells. Meta-topolin (mT) is increasingly been recommended in plant tissue culture studies because of its ability to support spontaneous root formation in the regenerating shoots, thereby reducing the time span for complete plantlet development. The present study describes a protocol for enhanced shoot regeneration and plantlet development of Sansevieria cylindrica with an application of mT as compared to other cytokinins viz., benzyladenine (BA), kinetin (Kn) and adenine sulphate (AdS). Higher shoot regeneration (mean number of 32.6 ± 0.40shoot per explant) with simultaneous root formation was achieved on Murashige and Skoog (MS) medium + mT (12.5 µM) after 6 weeks of culture. The media containing routine cytokinins (CKs) also responded well with a maximum of 12.6 ± 0.67shoots per explant on MS medium + BA (10.0 µM) but without spontaneous rooting and comparatively exhibited lesser growth and leaf development than mT containing medium after 6 weeks of culture. The micro shoots regenerated on BA containing medium were excised and rooted best on half-strength MS medium containing IBA (5.0 µM). The plantlets produced on mT containing medium survived better (90%) than those obtained on BA containing medium (67%) when acclimatized in garden soil + coco peat (1:1) after 4 weeks of acclimatization.
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How to cite this article:
Arjumend Shaheen, Najat A Bukhari, Kahkashan Perveen, Taiba Saeed, Anwar Shahzad. Meta-topolin influenced shoot morphogenesis in Sansevieria cylindrica Bojer ex Hook. through leaf disc culture. International Journal of Botany Studies, Volume 7, Issue 3, 2022, Pages 88-95
International Journal of Botany Studies International Journal of Botany Studies