Physalis minima L. a pubescent annual herb possess both horticultural as well as medicinal properties. The present study describes a reproducible protocol for high frequency in vitro shoot production through nodal explants and shoots tip explants when cultured on Murashige and Skoog (MS) medium supplemented with different Cytokinins. Optimum response was achieved through Shoot tip explants when cultured on 10.0 µM BA + 0.5 µM NAA which in turn resulted in the production of a maximum number of 77.4±0.50 adventitious shoots with a mean shoot length of 1.42±0.03 cm. Gibberellic Acid (GA) resulted in further proliferation and elongation when added to the optimized cytokinin concentration. Rooting was observed on half-strength MS medium fortified with different auxins either singly or in combination. Regenerated plantlets were successfully acclimatized, hardened and then transferred to green house with 90% survival rate.