Phytochemical screening showed the presence of biologically active phytochemical Flavonoids, Tannin, Glycosides, Triterpinoids, Steroids. Antibacterial activity of powder of Hibiscus rosa-sinensis was evaluated using disc diffusion and method. The zone of inhibition of hibiscus rosasinensis show better antibacterial activity against four pathogen like positive pathogen Staphylococcus aureus, Bacillus Subtilis and negative pathogen Pseudomonas Aeruginose and Escherichia coli.