The present paper deals with the investigations carried out to study the prevalence of fungal spores over Maize (Zea mays L.) fields during Kharif season in Niphad tehsil of Nashik district, Maharashtra, India and the coincidence of the disease development along with certain environmental parameters. The investigations carried out with the help of Tilak air sampler revealed the presence of certain airborne pathogenic fungi were responsible for causing diseases in certain crops some of them are, Anthracnose leaf blight (Colletotrichum), Curvularia leaf spot (Curvularia), Southern corn leaf blight (Bipolaris maydis or Drechslera), Gray leaf spot (Cercospora), and Common corn rust (Rust spore). The present investigation is beneficial for local grower to efficient control of diseases of crop plant at different developmental stages and improvement of yield and quality of crop.