Objective: This paper discuss the analytical strategies documented in the literature for the estimation of active compounds and vitamins in Moringa oleifera leaves by HPLC (High Performance liquid chromatography), HPTLC (High performance thin layer chromatography) Materials and Procedures: Widely used mobile phases and stationary phases in the literature. In HPTLC -Toluene: ethyl acetate: formic acid, Glass-backed silica gel plates 60F254-RP18 and 60F254. In HPLC- Methanol: Glacial acetic acid: water and Methanol: Water, C18 Column. Conclusion: Validated HPLC, HPTLC Techniques correctly employed for Quantification of vitamins and active components of Moringa oleifera leaves. These analytical techniques are cost effective and took less time for the evaluation.