Phytochemical and molecular profiling of tissue culture derived Bacopa monnieri (L.) Wettst clones against field grown plants
Debleena Roy, Dipu Samanta, Renia Mullick
There has been overexploitation of Bacopa monnieri from natural habitats due to growing commercialization and urbanization. Inspite of being found in the wetlands, the plant has a very low drug content i.e., 0.2% and hence for extraction of these drugs, large amount of plant material is needed which can be accomplished via tissue culture. Phenylalanine when used as a precursor in optimum concentration (0.25 – 0.5 mg/ml) showed rapid enhancement of the phytoconstituents in Brahmi. Upon protein profiling, maximum protein content was found in the tissue culture produced samples in comparison to the field grown ones.