International Journal of Botany Studies

International Journal of Botany Studies


International Journal of Botany Studies
International Journal of Botany Studies
Vol. 7, Issue 4 (2022)

Biologically active metabolites of endophytic Diaporthe brasiliensis species from Cassia fitula L


Jawed Shaikh, Ashfaque M Khan, Avinash Gawade, M M V Baig

Endophytic microorganisms are impending source of bioactive molecules and have shown proved medicinal applications. Modern age techniques involving the use of different organisms living in plant tissues root back in ancient times where leaf extracts were used. Endophyts act as the treasure house of number of biologically active metabolites having proved medicinal values. A crude ethyle acetate extract of the endophytic fungus Diaporthe brasiliensis, isolated from the medicinal plant Cassia fistula L, was examined for antibacterial and -amylase inhibitory activities in order to study the biological potential of endophytic fungi. Furthermore, bioactive metabolites have been investigated using analytical technique GC-MS. It was observed that Crude extract of Diaporthe brasiliensis showed antibacterial activity at two different concentration 50 and 100µg/l selectively against gram positive bacteria S. aureus and B. subtilis. Whereas negative result was observed against gram negative bacteria. Further, crude extract of Diaporthe brasiliensis was evaluated for α-amylase inhibitory potential which showed antidiabetic potential as like standard molecule acarbose. GC-MS analysis was revealed that crude extract contains various metabolites two molecules 7,9-di-tert-butyl-1-oxaspiro (4,5) deca 6,9-diene and 2,8-dione cholesta-4,6-dien-3-ol, (3beta) whose probable concentration was more than the other molecule.
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How to cite this article:
Jawed Shaikh, Ashfaque M Khan, Avinash Gawade, M M V Baig. Biologically active metabolites of endophytic Diaporthe brasiliensis species from Cassia fitula L. International Journal of Botany Studies, Volume 7, Issue 4, 2022, Pages 150-155
International Journal of Botany Studies