Banana is one of India's and the world's most important foods and economic crops. Meloidogyne sp., a burrowing worm, is a severe threat to banana Musa sp. production. Meloidogyne sp. resistance is absent in all commercial banana cultivars. The goal was to create a biopesticide for treating Meloidogyne infection on banana roots. Plant-parasitic nematodes are parasitic nematodes that feed on plants. Meloidogyne sp. was the most common plant-parasitic nematode in the Trichy and Karur districts. Biopesticide remains effective in preventing Meloidogyne sp. infection in a concentration-dependent manner. We used biopesticides derived from Cascabela thevetia, Datura stramonium, Calotropis gigantean and Abrus precatorius to suppress the M. incognita infection in bananas. This herbal biopesticide had high mortality rate in 500 μg/ml concentration.