Plants are utilised to treat a variety of infectious disorders, and their therapeutic efficacy is attributed to the existence of bioactive secondary metabolites with antibacterial capabilities. The present study reports on the bactericidal properties of Eleagnus conferta Roxb. an endangered and indigenous tree of the Western Ghats. The presence of phenolics, alkaloids, and terpenoids in the seed, leaf, and stem bark sequential extracts has been examined qualitatively and quantitatively. All of the extracts have been tested for their bactericidal properties. against human pathogenic bacterial strains viz. Staphylococcus aureus, Streptococcus pneumoniae, Vibrio cholera, Pseudomonas aeruginosa, Salmonella typhi and Klebsiella pneumoniae using agar well diffusion method. The MIC value was obtained using the agar well diffusion method and the Resazurin Micro Titer Plate Method. In leaf ethanol extract, the bactericidal property was well displayed against Streptococcus pneumoniae, with a considerable zone of inhibition, followed by Klebsiella pneumoniae. Seed ethanol extract had a considerable zone of inhibition against Vibrio cholera, and stem bark ethanol extract had bactericidal activity against Pseudomonas aeruginosa. The findings of this study back up the traditional applications of Eleagnus conferta leaves extract to treat pneumonia and bronchial diseases, seed extract to treat diarrhea, and stem bark extract to treat diabetic pus sores.