Physalis peruviana L. (Solanaceae) is a semi-shrub and semi-perennial plant with great potential for use in the cultivation of its fruit under greenhouse and open-field conditions. Besides that it has also the potential to be the rootstock of scion for other Solanaceous plants against pests and diseases. It is possible to set their plantation via seedlings from seed or cuttings. The most important problems to obtain rooted and uniform cuttings are the high mortality and the least rooting rates that mightily depend on plant types, cutting length and nursery practices applied. In the study, the effect of cutting length on obtaining healthy cutting-derived seedling of P. peruviana was studied. For this aim main stem derived branch cuttings in four lengths as 5 cm, 10 cm, 15 cm, and 20 cm were used. At the end of the 60th day, important cutting parameters (Rates of vitality, mortality, callusing, rooting and shooting; length of root; weights of root and shoot; root:shoot ratio ) were calculated. The data showed that the mortality rate of 5 cm cuttings (93.3%) is the highest and the other cutting lengths showed no differences in vitality, and rooting rate. Root weight and shooting rate were maximum in 10 cm cutting with 2.07 g/cutting and 53.3% respectively. Although callusing rate, root length and root:shoot ratio did not show statistical differences in cutting length, cuttings in 10 cm gave the highest results as 30% in callusing, 21.6 cm/cutting in root length, and 1.27 in root:shoot ratio. The results showed that in the vegetative cutting step of Physalis peruviana, 10 cm cuttings can be preferable to longer and shorter cuttings according to the study.