Plants have been used for health care at family circle level both in processed and un- processed form since remote past. Indigenous knowledge lay out basis for local level decision making in natural resources conservation in rural communities. However, until recently little has been done to explore for indigenous knowledge applied in care medicinal plants. In fact, the present study was the first set out to explore medicinal plants of Pathukani, Kanyakumari district in Tamil Nadu, India. This paper pen drive to focus on indigenous knowledge used in natural resources; particularly medicinal plants in local tribal people of Pathukani, Kanyakumari district in Tamil Nadu, India. Results revealed that, selective harvesting of medicinal plant parts was popular by 94% of the respondents, followed by practice of medicinal plant species. The study findings indicated that fifty plant species belonging to 46 genera and 27 families are documented that are used by the Kani people. Plant species of Solanaceae is predominantly used, followed by Apocynaceae, Lamiaceae, Malvaceae etc. All of the species grow wild. The traditional herbal healers quiet frequently uses leaves (19) followed by root (13), whole plant (11), fruit (6), seed (2), epicarp, latex and bulb (1 each). The most common method of drug preparation and application is the paste form (24 out of 86 applications), followed by decoction (19 applications), powder (8 applications) poultice and juicer (1 application each). To conclude, the defendant showed to possess sound indigenous knowledge on conservation and their knowledge varied based on their socio-demographic attributes. In view of these findings, the study recommends for safe guarding and furtherance of aboriginal knowledge of conservation across the community. Likewise, there is a need of disseminate indigenous knowledge on conservation by the elders to the youth. It was concluded that study area is rich in medicinal plants species and corresponding traditional knowledge. However, pull up the entire plant for ethno-medicine is a big warning to conservation of medicinal plants diversity in the study area.