Phenolic compounds have been shown to express bioactive properties and in particular antioxidant effects. In present investigation evaluation of total phenolics, total flavonoids and radical scavenging activity was studied in various parts (stem, root, leaf) of Rubia cordifolia (Rubiaceae) collected from three different localities viz. Panhala, Borbet and Patgaon. The highest values for total phenolics and flavonoid contents were recorded to be 42.56 mg GAE/ g FW and 15.56 mg RE/g FW respectively in leaf extracts of R. cordifolia. The radical scavenging activity was also found to be highest in the leaf extracts (95.74 %). The phenolics and flavonoids correlated significantly with its antioxidant capacity, contributing to its antioxidant potential. The results obtained in the present study signify the importance of R. cordifolia as a potential source of natural antioxidant.