Medicinal plants are very interesting, began to focus on discovering natural products as potential drugs against various ailments. In this study proposes to evaluate the antibacterial and larvicidal activities of selected plant Biophytum sensitivum (L.). Antibacterial activity has been tested against human pathogenic E. coli, Pseudomonas aeruginosa, Salmonella typhi, Staphylococcus aureus and Proteus vulgaris. The highest value observed from ethanol extracts of Biophytum sensitivum (L.) showed antibacterial activity against Escherichia coli. All the tested human pathogens were highly sensitive to ciprofloxacin. The N-butyl alcohol extracts of Biophytum sensiivum showed the best results of larvicidal activity against Aedes aegypti mosquito larvae. This study Biophytum sensitivum in the traditional system of to treat various infectious diseases, caused by the microbes as well as in mosquitos.