Colour is what the eyes see when light is reflected off an object. Dyes are coloured substances which impart colour when applied to a substrate. This phenomenon is known as dyeing. The colouring chemicals are called stains. The types of the stains to be used depend upon the chemical nature of the material, pH value of the fixative and the reactivity of the stain with the material. Staining is the colouring of the fixed or sectioned material with suitable organic or inorganic dyes to brighten the contrast between different structures. Natural dyes are obtained from different plant parts like fruits, leaf, bark, roots and stem. The use of natural dyes as an alternative to chemically synthesized dyes has great importance. The present study investigates extracting plant dyes from the fruit of Beta vulgaris and the leaves of Tectona grandis and tested for its effect on staining with stem sections of dicot, monocot. The effectiveness of natural mordants in the process of dyeing was also investigated using fruit extract of Citrus aurantifolia. Very thin transverse sections were kept in the dye extract for 2-5 minutes and then passed through mordant solution. Excellent differential staining was noticed on sclerenchyma, collenchyma and xylem tissues of stem sections, but the cortex, medulla and pith region occupied by parenchyma were stained less effectively. More intensity in colour appearance on sclerenchyma tissues with mordant of Citrus aurantifolia was noticed. Naturally obtained dyes from plants are less expressive and have no side effects.