This study was conducted in Wadi Al-Akhthar in the north-western region of Saudi Arabia. The main objective is to determine the floristic composition of the area, including a breakdown of its different life forms and the chorology of the recorded species. A total of 82 species belonging to 63 genera and 25 families of the Angiospermae were recorded. About 46.34% of the recorded species in the study area are belonging to three families: Asteraceae, Brassicaceae, and Fabaceae. The data also reflect a high degree of monotypism, where 56% of the recorded families were represented by a single species, and 60.78% of the genera were monotypic. Perennial species dominated the plant cover (61%) defining the character of the vegetation, while annuals were represented by about 39%. Chamaephytes (35.4) and Therophytes (31.7) were the most frequent lifeforms. Chorological analysis of the floristic data revealed that the monoregional Saharo-Arabian chorotype is the most dominant chorotype in Wadi Al-Akhthar (39%), followed by the monoregional Sudanian chorotype (11%). The results also showed the predominance of mono-regional taxa (63.4%) over the other phytogeographical elements followed by the bi-regional (30.5%). Information from the current study may provide a valuable reference for appropriate conservation and management of the study area.