Urease responsible for the rapid hydrolysis of urea to ammonia & CO2 plays crucial role in the persistent habitation of Helicobacter pylori which causes various gastrointestinal diseases such as gastritis, duodenal, peptic ulcer, gastric cancer, pyelonephritis etc. Plant-based natural products are therefore being utilised to cure disorders induced by urease enzyme. In the present investigation, inhibitory effects of different extracts prepared in different solvents (methanol, acetone & aqueous) of Rhododendron arboreum and R. campanulatum were examined against jack bean urease at a concentration range of 0.2-1.0 mg/mL. R. arboreum showed 61.10±0.33, 44.20±0.50 and 45.70±0.50% inhibition at a concentration of 1 mg/mL for methanol, acetone and aqueous leaf extracts respectively. On the other hand, R. campanulatum exhibited 51.45±2.30, 38.05±1.50 and 32.80±0.45% α-amylase inhibition for methanol, acetone and aqueous extracts respectively at 1 mg/mL. The inhibitory potential/activity increased with increasing concentration of each plant extract. The results further revealed that the methanol extracts of both the plants exhibited maximum inhibitory effects than other solvent extracts. This tends to show that the active metabolites or phyto-constituents of the different plant parts are better extracted with methanol than in other solvents. Therefore, the present study approves the medicinal value of these plants and scientifically validates them for use as a component of medicinal preparations for curing diseases associated with urease enzyme.