Ficus benghalensis L., (Moraceae) which is referred to as India’s National Tree that signifies spiritual knowledge and eternal life has been used in traditional medicinal practices such as Ayurveda, Unani and Siddha. All parts of the tree such as fruits, aerial roots, bark and leaves are known to be traditionally associated with diarrhea, dysentery, menorrhagia and nervous disorders. Older banyan trees are characterized by aerial prop roots that mature into thick, woody trunks, which can become indistinguishable from the primary trunk with age. Old trees can spread laterally by using these prop roots to grow over a wide area. In some species, the prop roots develop over a considerable area that resembles a grove of trees, with every trunk connected directly or indirectly to the primary trunk. This experimental plant was investigated for larvicidal activity. The fourth instar larvae of Aedes aegypti and Culex quinquefasciatus were exposed to test concentrations of 1000,750, 500,250 µg of hexane, chloroform: methanol [1:2] and ethanol extracts of prop root in dimethyl sulphoxide. The chloroform: methanol [1:2] extract showed 91% mortality after 48 hrs of incubation against the Aedes, LC50 of 0.302 followed by 0.219 and 0.214 for 750,500, and 250 µl concentrations respectively. Hexane extracts exhibited 95% mortality against Culex larvae. The formulations proved to be effective in inhibiting the metamorphosis. This research leads to possible utilization of new phytochemical compounds and their role in the near future as eco-friendly natural pesticide.