Agaricaceae is a family of edible mushrooms that have rich source of bioactive metabolies. The main objectives of this work were to check out the relative abundances of phytochemical constituents of dried fruiting bodies of Agaricus litoralis. The screening was accomplished for alkaloids, phenolic compounds, sterols, triterpenoids, coumarins and Carbohydrates. The color strength or the precipitate formation was used as analytical answers to these tests. The results of preliminary phytochemical screening showed that A. litoralis contained various metabolites: Alkaloids, flavonoids, tannins, sterols, triterpenoids, carbohydrates etc. Aqueous and organic extracts (n-hexane and CH2Cl2) of this mushroom were also exposed to preliminary antibacterial activity screening by disc diffusion method and found to be active against some selected bacterial and fungal strains. The organic extracts (n-hexane and CH2Cl2) of A. litoralis were exhibited highest antibacterial activity against both selected bacteria (Pseudomonas aeruginosa and Bacillus subtilis). The aqueous organic extracts have high antifungal activity against one fungal specie (Fusarium oxysporium).