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VOL. 8, ISSUE 11 (2023)
Assessment of genetic fidelity in vitro regenerated plantlets of Zingiber zerumbet (L.) Roscoe ex Sm
Authors
A Shiva Kumar, S K Reddy
Abstract
In the given study, we established an efficient and reproducible in
vitro plant regeneration protocol of Zingiber zerumbet Smith developed
from rhizome explants. The shoot bud explant developed from rhizome produced
the highest number of shoots (20 shoots/ explant) on medium amended with 1 mg-1L
BAP, 2 mg-1L TDZ and 0.4 mg-1L
NAA. Approximately 80% of shoots were induced simultaneously rooting on shoot
induction media itself i.e., MS media fortified with 1 mg-1L BAP, 2
mg-1L TDZ and 0.4 mg-1L
NAA. These plantlets were acclimatized under greenhouse conditions, and the
survival rate was recorded as 90%. Micropropagated plantlets thus obtained were
seemed to be morphologically similar to that of mother plants. In this study, Random
Amplified Polymorphic DNA (RAPD) molecular markers were deployed to determine
the genetic stability of plants obtained from the tissue culture of the Z.
zerumbet. Our findings using molecular markers revealed the genetic
uniformity of all in vitro regenerants were similar to their mother
plant. Thus this in vitro regeneration procedure could be a helpful
method for the genetic improvement of Z. zerumbet.
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Pages:40-44
How to cite this article:
A Shiva Kumar, S K Reddy "Assessment of genetic fidelity <i>in vitro</i> regenerated plantlets of <i>Zingiber zerumbet </i>(L.) Roscoe ex Sm". International Journal of Botany Studies, Vol 8, Issue 11, 2023, Pages 40-44
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