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VOL. 8, ISSUE 12 (2023)
Assessment of genetic fidelity in vitro regenerated plantlets of curcuma amada Roxb
Authors
Swapna Nareddy, S K Reddy
Abstract
A highly efficient and reproducible in
vitro plant regeneration method has been developed from rhizome explants of
Curcuma amada Roxb. The shoot bud explant developed from rhizome produced the
highest number of shoots (16 shoots/ explant) on medium amended with 1 mg/L
BAP, 1 mg/L TDZ and 0.2 mg/L NAA. Approximately 80% of shoots were induced
spontaneous rooting on shoot induction media fortified with 0.2 mg/L NAA. The
plantlets were acclimatized under greenhouse conditions, and the survival rate
was recorded as 96%. The plantlets obtained from all explants were
morphologically similar to mother plants. Random Amplified Polymorphic DNA
(RAPD) molecular markers were employed to determine the genetic stability of
plants obtained from the tissue culture of the Curcuma amada Roxb. The DNA
markers validated the genetic uniformity of the plants obtained from all in
vitro regenerants was similar to their mother plant. This plant
regeneration procedure could be a helpful method for the genetic improvement of
Curcuma amada Roxb.
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Pages:1-5
How to cite this article:
Swapna Nareddy, S K Reddy "Assessment of genetic fidelity <i>in vitro</i> regenerated plantlets of curcuma amada Roxb". International Journal of Botany Studies, Vol 8, Issue 12, 2023, Pages 1-5
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