ARCHIVES
VOL. 8, ISSUE 12 (2023)
Evaluation of the cytotoxicity of the insecticide profen super by pollen germination assay
Authors
Madhu Bajaj, Saloni Bahri, Renuka Agrawal, Somdutta Sinha Roy, Mishthi Khurana3, Yamini Sharma
Abstract
The yield of cultivated plants is greatly reduced due to attack by
different kinds of pests. Application of pesticides to destroy the pests is a
common agricultural practice. The in vitro
pollen germination assay has been used to demonstrate the toxicity of many
chemical compounds. As pollen cultures do not require aseptic conditions and
are easy to set up, pollen grains offer a sensitive and easy assay system for evaluating
the cytotoxicity of chemicals which can be incorporated in the germination
medium. The parameters used to indicate cytotoxicity of the chemical are inhibition
of germination of pollen and elongation of pollen tube. In the present study,
the cytotoxicity of Profen Super, an insecticide, was assessed by performing
the pollen germination assay in two plant species namely, Catharanthus
roseus (L.) G. Don. and Clitoria ternatea L. Four dilutions (1:1,
1:2, 1:4 and 1:8) of the recommended dose of Profen Super were added in the
germination medium used to culture pollen grains. The results clearly show that
all tested dilutions of this insecticide are cytotoxic. Thus, the use of this
insecticide should be avoided or a safe concentration should be determined
which would allow fruit and seed set simultaneously with eradication of the
pest.
Download
Pages:53-59
How to cite this article:
Madhu Bajaj, Saloni Bahri, Renuka Agrawal, Somdutta Sinha Roy, Mishthi Khurana3, Yamini Sharma "Evaluation of the cytotoxicity of the insecticide profen super by pollen germination assay". International Journal of Botany Studies, Vol 8, Issue 12, 2023, Pages 53-59
Download Author Certificate
Please enter the email address corresponding to this article submission to download your certificate.