Dragon fruit is considered an important economic fruit species worldwide due to its rich nutrient contents and strong to lerance to drought stress. In order investigate Genetic Analysis of Dragon fruits in Telangana 6 Germplasms were subjected to RAPD. The size of RAPD bands measured and score. The RAPD bands were scored as 1 if present and 0 if absent of bands of same molecular weight Five primers (Df series) amplified a total length of 39 loci with a size of 150-1400 bp. Amplified from the genomic DNA of Germplasm using 25 Decamer primers of random sequence were initially screened on DNA samples of 6 dragon fruit germplasm to evaluate their sustainability for amplifying DNA sequences, which could be accurately scored. A final subset of 5 primers.