Cyanobacteria are considered as important members of ecosystem as they have immense potential for creating sustainable agriculture output. The prime existence in the rice fields is very well established due to many emerging benefits such as enhanced soil fertility, nitrogen fixation, P solubilisation and of course increased yields. Present study has screened many cyanobacteial species from various locations of Trimbakeshwar, Maharashtra. They have found majorly Oscillatoria simplicissima (5%) followed by Anabaena constricta (4.6%), Nostoc spongiaeforme (4.2%) species. They detected relative abundance of 7.4% in Pahine and Deogaon while minimum was found in Ghytipada, Behedpada, Harsul with RA of 3.1%. Furthermore Deogaon was found to be rich in cyanobacterial population with the highest Shannon index (4.0) followed by Alwand (2.7) and Pahine (2.65). Thus study put a light on the importance of cyanobacterial species in rice filed which could be an important candidate for agricultural revolution in future.