Plants are the storehouse of a large number of pharmacologically important compounds having antioxidant activities. Salvadora persica, commonly called miswak plant, is well documented for its antidiabetic, antihyperlipidemic and antimicrobial activities. In the present study antioxidant activity of five different extracts of S. persica stem along with total phenolic content were investigated by different antioxidant assays that include free radical (DPPH, superoxide, hydroxyl) scavenging activity, reducing power assay and metal chelating capacity. Total phenolic content in methanolic extract was 32.50 mg/g gallic acid equivalent. The results depicted that methanolic extract possessed the highest antioxidant activity which gets enhanced with increasing concentration. Antioxidant activities showed good correlation with total phenolic content suggesting the role of phenolics in quenching free radicals. These results indicate that methanolic extract of S. persica stem displayed significant antioxidant activities establishing its role as source of natural antioxidants.