The present study deals with the estimation of potential of cyanobacteria for nitrogen fixation in paddy field of Trimbakeshwar area. Water and soil sample were collected from different site and were used for identification and isolation of cyanobacterial species. Ocular micrometry method was used for identification of species classified under cyanobacteria. Thus Calothrix, Nostoc, Anabeana, And Oscillotoria Sp. Was identified and used for study purpose. These species were isolated in BG-11 media for growth. Further the nitrogen content was estimated by using the famous Kjeldahl method. According to the result obtained Anabeana have the highest capability of Nitrogen fixation (71.05 mg/l) followed by Oscillotoria (50.51 mg/lit), then Nostoc (68.80 mg/lit) and the minimum Nitrogen fixation was observed for the Calothix species (47.00 mg/lit). For agricultural growth, the Anabeana can be used as a biofertilizer. Moreover, Nostoc Sp., Oscillotoria Sp., and Calothrix Sp. have demonstrated the ability to fix nitrogen and can take the place of Anabeana sp. As a result, the study might draw the conclusion that harmful chemical fertiliser can be replaced with nitrogen-fixing cyanobacterial species.