The plant Solanum nigrum L. traditionally claimed in the treatment of mouth ulcers and is used in cooking like spinach. The main aim of the present work is to investigate the phytoconstituents by Liquid Chromatography - Mass Spectrometry (LC-MS) analysis. For analysis hydroethanolic extracts were used. The spectrum interpretation and fragmentations identification were carried out using a spectrum database for organic compounds in SDBS application. They showed the presence of five and seven constituents in variant black and orange fruits respectively. The analysis revealed the presence of same phytoconstituents like Solasonine, Solamargine, and Triterpenoids derivatives in both variants.