The quantitative distributions of two major quaternary ammonium compounds betaine and choline were obtained by the use of both the specific but semi quantitative Thin Layer Chromatography method and the sensitive quantitative but less specific periodide assay. Lentil plants accumulated two organic metabolites betaine and choline not only control plants but also when exposed salt stress. The maximum betaine concentration (0.416 g/gm wt.), attained by mature plants of var.’PL-4’ at 4mScm-1 EC, was apparently higher than of choline concentration (0.695 g/gm dry wt.) attained by mature plants of var. ‘PL-406’. However, the rate of accumulation of both betaine and choline appeared to be the same in all varieties of lentil investigated.