ARCHIVES
VOL. 8, ISSUE 5 (2023)
In vitro propagation and PLBs multiplication of Eulophia graminea Lindl. on MS medium supplemented with different additives or PGRs
Authors
Tapash Kumar Bhowmik, Md Mahbubur Rahman
Abstract
Eulophia graminea
Lindl. a terrestrial medicinal orchid occasinally found in Chittagong Hill
Tracts and the seeds were cultured on 0.8% (w/v) agar solidified MS medium
supplemented with eight different types of additives viz. activated
charcoal, banana powder, peptone, vanilla powder, amaranth juice, coconut
water, pineapple juice and tomato juice. The required minimum time for
initiation of seed germination (9.36 ± 0.34 wks) and induction of protocorms (15.21
± 0.57 wks) was recorded on MS medium fortified with 2 g/l peptone. For the
development of seedlings, the lowest required time was 31.50 ± 0.56 wks on MS
medium supplemented with 15% CW. The highest percentage (93.34%) of seed
germination was achieved on MS medium supplemented with 1 g/l AC, 2 g/l P, 20%
CW. The highest increase of PLBs multiplication was observed on MS medium
fortified with 2.0 mg/l 2,4-D + 0.8 mg/l BAP after 30d (0.78 ± 0.04 g, 37.2 ±
0.86 no) and 60d (1.94 ± 0.04 g, 87.4 ± 0.51 no) of culture respectively. The
maximum increase in length (3.52 ± 0.03 cm) and number of roots (4.50 ± 0.22
no) were induced in seed originated plantlets on MS medium with 1.0 mg/l IBA +
1.0 mg/l NAA. The hardened seedlings were successfully transplanted in outside
green house condition.
Download
Pages:11-17
How to cite this article:
Tapash Kumar Bhowmik, Md Mahbubur Rahman "<i>In vitro</i> propagation and PLBs multiplication of <i>Eulophia graminea</i> Lindl. on MS medium supplemented with different additives or PGRs". International Journal of Botany Studies, Vol 8, Issue 5, 2023, Pages 11-17
Download Author Certificate
Please enter the email address corresponding to this article submission to download your certificate.