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VOL. 8, ISSUE 7 (2023)
Pectolytic enzymatic activity and phenolic response of leaf spot pathogen from sugarcane (Saccharum officinarum) under controlled nutritional condition
Authors
Dr. Abhijeet B Pawar, Dr. Darshsn M Kokate
Abstract
Sugarcane (Saccharum officinarum) is a major commercial crop
affected by various biotic stresses, among which foliar pathogens like Helminthosporium
tetramera play a significant role in yield reduction. This study aimed to
evaluate the pectolytic enzyme activity and phenolic response of H.
tetramera under different nutritional conditions using Czapek-Dox medium
amended with varied carbon and nitrogen sources. The enzymatic assay, based on
the maceration of potato discs, was conducted to determine the macerating
enzyme (ME) activity, while total phenolic content in culture filtrates was
estimated using the Folin–Ciocalteu method. Results demonstrated that
among carbon sources, fructose supported the highest pectolytic activity (ME =
200), whereas lactose enhanced phenolic production (3.595 mg/L). Nitrogen
source Co(NO₃)₂ significantly induced both macerating enzyme activity (ME =
166.66) and phenol production (3.225 mg/L). The differential enzymatic
expression and phenolic secretion suggest an adaptive metabolic response of H.
tetramera influenced by nutritional composition
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Pages:36-38
How to cite this article:
Dr. Abhijeet B Pawar, Dr. Darshsn M Kokate "Pectolytic enzymatic activity and phenolic response of leaf spot pathogen from sugarcane (<i>Saccharum officinarum</i>) under controlled nutritional condition". International Journal of Botany Studies, Vol 8, Issue 7, 2023, Pages 36-38
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