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VOL. 8, ISSUE 7 (2023)
A comparative study of oxidative stress biomarkers in flowers and fruits during senescence
Authors
Janaki Subramanyan, Rajeshwari, Diksha Patel, Pallavi Sharma
Abstract
Azadirachta indica, Delonix regia and Murraya koenigii are drought-tolerant trees, and Bougainvillea
glabra is a heat- and drought-tolerant shrub. In this study extracts
prepared from fresh and senescing flowers of B. glabra including the
bracts, petals of fresh and senescing flowers of D. regia, and the young
and mature fruits of A. indica and M. koenigii excluding the
seeds have been compared for their pigments, and the activities of the enzymes
catalase and oxidase. Catalase activity was studied by using 1% hydrogen
peroxide as the substrate; the oxygen evolved was measured 5 minutes from
incubation. Using catechol (1%) as the substrate, oxidase activity was compared
by observing the intensity of browning of the reaction mixture. The comet assay
was performed in order to find out if there was any DNA damage in bracts of
senescing flowers of B. glabra. The chlorophyll a, chlorophyll b and carotenoid
contents were more in senescing flowers compared to fresh flowers in both B.
glabra and D. regia. Betacyanin was present in senescing flowers of B.
glabra but absent in fresh flowers. The amount of anthocyanins was more in
senescing flowers of D. regia compared to that in fresh flowers. Fresh
flowers of B. glabra showed catalase activity and the activity decreased
as the flowers senesced. Fresh flowers of D. regia did not show catalase
activity whereas the senescing flowers showed a low catalase activity.
Catalase activity was more in mature fruits than in young fruits in the two
taxa studied. Oxidase activity was detected in all the experimental materials
studied. Fresh flowers of B. glabra had more oxidase activity than
senescing flowers. The oxidase activity was the same in fresh and
senescing flowers of D. regia. Oxidase activity was more in mature
fruits than in young fruits. No damage to DNA was detected in the
nuclei of the bracts of senescing flowers of B. glabra when
the comet assay was performed.
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Pages:11-17
How to cite this article:
Janaki Subramanyan, Rajeshwari, Diksha Patel, Pallavi Sharma "A comparative study of oxidative stress biomarkers in flowers and fruits during senescence". International Journal of Botany Studies, Vol 8, Issue 7, 2023, Pages 11-17
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