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VOL. 8, ISSUE 9 (2023)
Isolation and identification of fungi associated with seeds of chickpea (Cicer arietinum L.)
Authors
Jitendra Kumar Rainkwar, Archana Singh
Abstract
Due to various fungal diseases, production of chickpea crop has been
decreased in recent years. In the present study, chickpea seeds were collected
from different districts of Rajasthan (Bharatpur, Alwar, Dholpur, Karauli and Kota).
These seeds were subjected for isolation and identification of the associated
fungi with this. Percent frequency of the associated fungi was also calculated.
For each seed sample, dry seed examination was done. Besides normal seeds, some
seeds had abnormal colour (blackish) (4-25%), seeds with white mycelial growth
(0.60-11%), shriveled seeds (2-8%) and damaged seeds (5-45%). In the present
study different fungi were found to be associated with chickpea seeds. Total 56
isolates were obtained which were identified morphologically as 16 species of
fungi. The isolated fungi were identified as Alternaria alternata, Fusarium
oxysporum, Aspergillus flavus, Penicillum species, Rhizopus sp., Botryodiplodia
theobromae, Curvularia lunata, Chaetomium globosum, Rhizoctonia solani,
Macrophomina phasaelina, Stemphylium sarciniforme, Aspergillus niger, Sclerotium rolfsii, Verticillium
sp., Cladosporium sp., and Negrospora
sp. Among all the associated fungi, 5 fungi i.e. Alternaria
alternata, Fusarium oxysporum, Aspergillus flavus, Penicillum species and Rhizopus
sp. were present in the maximum frequency.
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Pages:1-4
How to cite this article:
Jitendra Kumar Rainkwar, Archana Singh "Isolation and identification of fungi associated with seeds of chickpea (<i>Cicer arietinum</i> L.)". International Journal of Botany Studies, Vol 8, Issue 9, 2023, Pages 1-4
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