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VOL. 9, ISSUE 11 (2024)
Phytochemical screening and GC- MS analysis of Musa ornata pseudo stem
Authors
P Radha, S Karunakar Reddy
Abstract
The present investigation focuses on the phytochemistry of the Musa
ornata pseudo-stem using advanced analytical techniques. The ethanolic
extract obtained from Soxhlet extraction at 50°C for 48 h, is subjected to the
preliminary phytochemical screening which revealed the presence of
carbohydrates, proteins, steroids, saponins, flavonoids, and tannins. Thin
Layer Chromatography (TLC) performed using four different solvent systems:
ethyl acetate (1:9 v/v), ethyl acetate (2:8 v/v), toluene acetate acid
(4.5:3.5:0.2 v/v/v), and ethyl acetate acid (100:11:11:27 v/v/v/v). This
clearly pictured varying degrees of compound separation, with the best
resolution achieved using the most complex solvent system (Plate D). Gas
Chromatography-Mass Spectrometry (GC-MS) analysis was conducted using an
AOC-20i+S auto-injector and GC-2010 system. GC-MS analysis identified a diverse
range of compounds including (1R)-2,6,6-Trimethylbicyclo [3.1.1] hept-2-ene,
1,2,4-Trimethylbenzene, 2-(2-Ethylhexyloxy) ethanol, Nonanal, Dodec-1-ene,
Decanal, and 2,6-Di-tert-butylphenol. These compounds are of significant
interest in botany and phytochemistry due to their potential bioactivity and
role in the plant's physiological processes. Notable plasticizers such as
Dibutyl phthalate and Bis(2-ethylhexyl) phthalate were also detected. The
presence of phenolic and quinone compounds suggests potential antioxidant
properties, supporting the traditional medicinal use of Musa ornata and
indicating its therapeutic potential. However, further studies are required to
explore the biological activities of these compounds, underlining the importance
of this research.
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Pages:16-22
How to cite this article:
P Radha, S Karunakar Reddy "Phytochemical screening and GC- MS analysis of <i>Musa ornata</i> pseudo stem". International Journal of Botany Studies, Vol 9, Issue 11, 2024, Pages 16-22
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