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VOL. 9, ISSUE 12 (2024)
Micropropagation studies from nodal and internodal explants of Tylophora indica. (Burm. f.) Merrill
Authors
N Ramamurthy, S Balaraju, S Suresh
Abstract
This review provides an in-depth and comprehensive overview of the in
micropropagation studies of Tylophora species, which have medicinal
properties. It is a threatened medicinal plant (climber) of the family
Asclepiadaceae. Here in the present study we developed an efficient
micropropagation protocol from nodal and internodal explants of Tylophora
indica. micropropagation is an alternative method of propagation of
the threatened and endangered plant which can aid its conservation. The nodal
and inter-nodal explants were cultured on MS medium containing different
concentration and combinations of growth regulators like cytokines, 6- benzyl
amino purine (BAP) and Kn. Multiple shoot buds were regenerated successfully
from the nodal explants which were efficiently rooted on half strength MS
medium supplemented with 6- benzyl amino purine (BAP) and Indole- 3-butyric
acid (IBA). The regenerated plantlets were successfully transferred to the
glasshouse, acclimatized and transferred to the field.
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Pages:11-15
How to cite this article:
N Ramamurthy, S Balaraju, S Suresh "Micropropagation studies<i> </i>from nodal and internodal explants of <i>Tylophora indica.</i> (Burm. f.) Merrill". International Journal of Botany Studies, Vol 9, Issue 12, 2024, Pages 11-15
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