Primary metabolites, including total soluble sugars, proteins, and
lipids, are found abundantly in both plant species, with higher levels observed
in C. tora compared to R. patula. Although primarily associated
with plant growth and development, these metabolites play crucial roles in
stress tolerance and metabolic regulation. Secondary metabolites, such as total
phenols, flavonoids, alkaloids, tannins, and terpenoids, are detected in both
species, with C. tora exhibiting higher phenolic content and R. patula
showcasing elevated levels of flavonoids, alkaloids, and tannins. These
compounds fortify plant immunity and have been commercially exploited for their
therapeutic potential against oxidative and inflammatory conditions. Extracts
from the leaves of C. tora and R. patula exhibit potent
antioxidant activity, with R. patula demonstrating higher efficacy attributed
to its richer secondary metabolite profile. Moreover, these extracts display
significant antimicrobial properties against both Gram-positive and
Gram-negative bacteria. C. tora shows higher activity against E.
coli, while R. patula exhibits superior efficacy against B.
subtilis and P. aeruginosa.
The antimicrobial action is attributed to the presence of bioactive
secondary metabolites, including alkaloids, saponins, tannins, flavonoids,
phenolic compounds, and terpenoids, which disrupt bacterial growth and
virulence through various mechanisms. The findings
underscore the remarkable antioxidant and antibacterial potential of C. tora
and R. patula, positioning them as "wonder plants" in
combating oxidative stress and microbial infections. However, the study's scope
is limited to demonstrating the antioxidant and antibacterial effects of the
plant extracts, with molecular mechanisms remaining unexplored.
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