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VOL. 9, ISSUE 6 (2024)
Single and combined effects of synthetic auxin and cytokinin on in vitro microplants of sweet potato (Ipomoea batatas L.)
Authors
Abdur Rahim, Shakil Rahman Shoagh, Anika Tasnim Khan Mim, Baishakhi Roy, M Nasiruddin
Abstract
The sweet potato (Ipomea batatas L.) is vegetatively propagated
crop rich in vitamins and sugar. Producers avoid it because vegetative
development favors virus-infected materials, lowers yields, and increases input
needs. Virus-free microplants are possible but difficult through tissue culture.
This work evaluated a highly effective disease-free in vitro microplant
production protocol using MS media with BA, KIN alone, and together with NAA.
The present investigation all the hormonal tests have exhibited a consistent
and proportional increase over time (15 and 30 days respectively). The
character shoot initiation was early responsive in the T3 (2.00 mg-l)
of single treatment and T12 (3.00 mg-l BA with 0.5 mg-l
NAA), T14 (1.00 mg-l KIN with 0.5 mg-l NAA), T15 (2.00 mg-l
KIN with 0.5 mg-l NAA) of combined treatments and less responsive
was T6 (1.00 mg-l KIN) of single treatment. Among the combined
treatments optimum effect was observed in most of the studied characters with a
notable root growth in T7 (2.00 mg-l KIN), T8 (3.00 mg-l
KIN), T11 (2.00 mg-l BA with 0.5 mg–l NAA), T13 (4.00 mg-l
BA with 0.5 mg-l NAA) and T15 (2.00 mg-l KIN with 0.5 mg-l
NAA). Interestingly, the treatment T1 (control) was observed good quality of
every agronomic characters but root growth were absent. Thus, it should be
concluded that hormonal effect was affected by the in vitro microplants
growth and development of the sweet potato.
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Pages:33-38
How to cite this article:
Abdur Rahim, Shakil Rahman Shoagh, Anika Tasnim Khan Mim, Baishakhi Roy, M Nasiruddin "Single and combined effects of synthetic auxin and cytokinin on <i>in vitro</i> microplants of sweet potato (<i>Ipomoea batatas</i> L.)". International Journal of Botany Studies, Vol 9, Issue 6, 2024, Pages 33-38
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