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VOL. 9, ISSUE 8 (2024)
Synthesis of clindamycin conjugated silver nanoparticles using Plumbago Zeylanica stem extract and their antibacterial effect against multidrug resistant pathogenic bacteria
Authors
Malini BP, Raghu HS, Raghavendra SN, Rajeshwara Achur
Abstract
The problem of multidrug resistance (MDR) against commonly used
antibiotics is of growing concern globally. Novel and effective strategies are
being employed to treat various disease causing MDR pathogens. In the present
study, we have prepared silver nanoparticles (AgNPs) and antibiotic clindamycin
conjugated silver nanoparticles (Cm-AgNPs) by green synthesis using aqueous Plumbago
zeylanica leaf extract for their application to treat gram positive MDR
pathogens. The synthesized NPs were characterized by UV-visible spectroscopy,
Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy
(FT-IR) and X-ray diffraction analysis (XRD). The UV-visible spectra of AgNPs
and Cm-AgNPs show characteristic absorption peak at 440nm and 460nm respectively
confirms the formation of NPs. The distinct SEM images of AgNPs and
Cm-AgNPs show the formation of spherical particles without aggregation. The
FT-IR spectrum show corresponding absorption bands of 3890cm-1 and
3000cm-1 confirms the AgNPs, and the presence of absorption band at
1100cm-1 confirms the conjugation of antibiotic. The XRD pattern obtained for
the AgNPs and Cm-AgNPs show prominent Diffraction peaks with 2θ values confirms
the crystalline nature of NPs. The antimicrobial activity of AgNPs and Cm-AgNPs
were tested against three Gram positive MDR pathogens Enterococcus faecalis,
Enterococcus faecium and Staphylococcus aureus by agar well plate
method. Cm-AgNPs showed 7.14 %, 8 % and 8.33% more activity against E.
faecalis, E. faecium and S. aureus respectively, compared to
standard antibiotic Clindamycin. These results clearly demonstrate the
utility and potency of Cm-AgNPs in combating MDR gram positive pathogens.
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Pages:24-30
How to cite this article:
Malini BP, Raghu HS, Raghavendra SN, Rajeshwara Achur "Synthesis of clindamycin conjugated silver nanoparticles using <i>Plumbago Zeylanica</i> stem extract and their antibacterial effect against multidrug resistant pathogenic bacteria". International Journal of Botany Studies, Vol 9, Issue 8, 2024, Pages 24-30
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