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VOL. 9, ISSUE 8 (2024)
Effects of phytohormones on the growth and development of in vitro microplants of Mulberry (Morus alba L.)
Authors
Anika Tasnim Khan Mim, Abdur Rahim, Baishakhi Roy, Md. Shakil Rahman Shoagh, M Nasiruddin
Abstract
The ancient Mulberry (Morus alba L.) plant serves as a popular
silkworm feed, as well as a source of food for animals and medication.
Vegetative propagation can result in reduced yields of virus-infected
materials. This study aimed to evaluate the efficacy of a disease-free in
vitro microplant culture technique by testing several combinations of MS
media supplemented with BA, KIN alone, BA with NAA, and KIN with NAA. The
experiment required the use of explants obtained from fully developed plants
cultivated in the field, which were subjected to surface sterilization. After
several attempts, it was determined that the sterilization method employing a
0.1% HgCl2 solution was effective. Nodal explants performed better than
alternative techniques for axillary shoot proliferation. The results of this
experiment showed that BA (cytokinin) was superior to KIN (cytokinin) in terms
of axillary shoot production, and roots were well formed in KIN and NAA.
Auxillary shoot growth was shown to be tremendous on MS medium supplemented
with 2.5 mg/l BA (T4) and a combined hormone of 2.0 mg/l BA with 0.25 mg/l NAA
(T8), 1.5 mg/l KIN with 0.25 mg/l NAA (T11), and 1.0 mg/l KIN with
0.5 mg/l NAA. Moreover, significant root formation was found in KIN 2.5 mg/l in
collaboration with 0.5 mg/l NAA (T13). Consequently, it should be concluded
that the development and progression of the mulberry microplants had an impact
on the hormonal action.
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Pages:61-64
How to cite this article:
Anika Tasnim Khan Mim, Abdur Rahim, Baishakhi Roy, Md. Shakil Rahman Shoagh, M Nasiruddin "Effects of phytohormones on the growth and development of <i>in vitro</i> microplants of Mulberry (<i>Morus alba</i> L.)". International Journal of Botany Studies, Vol 9, Issue 8, 2024, Pages 61-64
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